![]() Delivery of growth factor from controlled release systems has been found to overcome these problems as they protect the growth factors from degradation and also result in sustained delivery of growth factors at the site of injury.Ĭhitosan has been used in the treatment of wounds because of its anti microbial, hemostatic and muco adhersive properties 5, 6, 7, 8, 9. Growth factors have also proved to have undesirable side effects at high systemic levels 4. They are also quickly degraded and inactivated by various factors at the site of injury. Local application of growth factors has been found to have poor efficiency due to their short half-life and rapid dilution in the body. Application of growth factors at the site of the wound has shown to improve the efficiency and quality of wound healing as they stimulate angiogenesis and proliferation of cells and that in turn regulates the production and degradation of the extracellular matrix 2, 3. Inflammatory cells and stromal cells secrete growth factors at the site of injury which helps in all phases of wound healing 1. Wound healing is a complex process which mainly consists of three phases like inflammation, proliferation and tissue remodeling. Thus, it can be a promising candidate for wound healing. Thus our system can maintain therapeutic levels of growth factor at the wound bed thereby avoiding the need for daily applications and frequent dressing changes. Our system was found to release both VEGF and bFGF in a continuous manner and attained stability after 7 days. Frequent dressing changes are a major deterrent to proper wound healing. Immunohistochemistry was performed using the Ki67 marker which increased proliferation of cells in growth factor containing scaffold treated group. The growth factor containing scaffolds induced increased proliferation of HaCaT cells, increased neovascularization and collagen formation seen by H and E and Masson’s trichrome staining. The goal of the study was to develop a Polyethylene glycol (PEG) cross-linked cotton-like chitosan scaffold (CS-PEG-H) by freeze-drying method and chemically conjugate heparin to the scaffold to which the growth factors can be electrostatically bound and evaluate its wound healing properties in vitro and in vivo. Delivery of growth factor from controlled release systems protect it from degradation and also result in sustained delivery of it at the site of injury. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) induce proliferation of various cells in wound healing. Application of growth factors at wound site has improved the efficiency and quality of healing. ![]()
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